Mechano-immunomodulation
Mechano-immunomodulation - Related Videos
09:07
Murine Model of Epicutaneously-Induced Immunomodulation
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2025
The study demonstrates the role of the skin in modulating immune responses in mice through epicutaneous (EC) immunization. EC immunization with a protein antigen can suppress immune responses in contact hypersensitivity or reverse skin-induced suppression when combined with pathogen-associated molecular patterns. EC immunization shows promise as an effective, needle-free approach to immunotherapy.
10:25
Easy and Accurate Mechano-profiling on Micropost Arrays
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Cited by 9 •
2015
Described are protocols for quantifying mechanical interactions between adherent cells and microstructured substrates. These interactions are closely linked to essential cell behaviors including migration, proliferation, differentiation, and apoptosis. The protocols present an open-source image analysis software called MechProfiler, which enables determination of involved forces for each micropost.
12:42
Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
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Cited by 17 •
2019
This protocol describes a detailed workflow for the generation and ex vivo characterization of oncolytic viruses for expression of immunomodulators, using measles viruses encoding bispecific T cell engagers as an example. Application and adaptation to other vector platforms and transgenes will accelerate the development of novel immunovirotherapeutics for clinical translation.
06:02
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
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Cited by 8 •
2018
Here, we present the protocols to identify 1) virus-encoded immunomodulators that promote arbovirus replication and 2) eukaryotic host factors that restrict arbovirus replication. These fluorescence- and luminescence-based methods allow researchers to rapidly obtain quantitative readouts of arbovirus replication in simplistic assays with low signal-to-noise ratios.
05:49
Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements
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Cited by 2 •
2022
Presented here is a method to mechanically phenotype single cells using an electronics-based microfluidic platform called mechano-node-pore sensing (mechano-NPS). This platform maintains moderate throughput of 1-10 cells/s while measuring both the elastic and viscous biophysical properties of cells.
FAQs
Integrins connect immune cells to their surrounding material or tissue, while the cytoskeleton distributes forces through the cell. Together, they help convert physical resistance or deformation into force-dependent signaling. This internal transmission can influence migration, activation, and cytokine production, allowing immune cells to respond differently to mechanically distinct microenvironments.
Each cue presents a distinct physical condition that cells can sense and integrate. Stiffness changes resistance from the surrounding environment, stretch deforms cells or tissues, fluid shear applies forces from moving fluid, and confinement restricts available space. These variables can alter mechanosensitive signaling and thereby change immune-cell movement, activation, or cytokine production.
Mechanosensitive ion channels provide a force-responsive route for converting changes in the cellular environment into signaling events. When mechanical conditions alter membrane or cellular forces, these channels can participate in pathways that regulate immune-cell behavior. Their involvement helps explain how physical microenvironments influence activation and cytokine production alongside integrins and cytoskeletal structures.
Bioengineered biomaterials can be used to reproduce or control the mechanical microenvironments encountered by immune cells. By presenting defined physical conditions, these materials support examination of how mechanics interact with cellular signaling and immune behavior. Such systems are useful for investigating inflammation and repair while testing how engineered surroundings may modulate responses.
Organ-on-chip systems are useful when researchers need an engineered platform that reproduces relevant mechanical microenvironments while examining immune behavior. They can support studies connecting physical cues with inflammation, tissue repair, infection, or disease processes. Their value comes from linking controlled bioengineering conditions to changes in immune-cell migration, activation, or cytokine production.
The framework helps researchers examine how tissue mechanics contribute to immune behavior across several biological settings. In fibrosis and cancer, it can connect altered physical environments with inflammation or disease-associated responses. In infection and regenerative medicine, engineered tissues and biomaterials can help study or control immune activity during host responses and repair.