Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Andre Lai; Rachel Rex; Kristen L. Cotner; Alan Dong; Michael Lustig; Lydia L. Sohn

doi:10.3791/64665

Journal

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Bioengineering

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Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

2,604 Views

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05:49 min

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December 02, 2022

DOI:

10.3791/64665-v

DOI:

10.3791/64665-v

•

05:49 min

December 02, 2022

•

2598 Views

Andre Lai*¹, Rachel Rex*², Kristen L. Cotner¹, Alan Dong³, Michael Lustig^1,3, Lydia L. Sohn^1,2

¹Graduate Program in Bioengineering,University of California, Berkeley and University of California, San Francisco ²Department of Mechanical Engineering,University of California, Berkeley ³Department of Electrical Engineering and Computer Sciences,University of California, Berkeley

Summary

Presented here is a method to mechanically phenotype single cells using an electronics-based microfluidic platform called mechano-node-pore sensing (mechano-NPS). This platform maintains moderate throughput of 1-10 cells/s while measuring both the elastic and viscous biophysical properties of cells.