Presented here is a method to mechanically phenotype single cells using an electronics-based microfluidic platform called mechano-node-pore sensing (mechano-NPS). This platform maintains moderate throughput of 1-10 cells/s while measuring both the elastic and viscous biophysical properties of cells.
Lai, A., Rex, R., Cotner, K. L., Dong, A., Lustig, M., Sohn, L. L. Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements. J. Vis. Exp. (190), e64665, doi:10.3791/64665 (2022).