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Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
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JoVE Journal Cancer Research
Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

DOI:
10.3791/58651-v

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12:42 min

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January 07, 2019

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,
1Department of Translational Oncology,German Cancer Research Center (DKFZ) and National Center for Tumor Diseases (NCT), 2Faculty of Biosciences,Heidelberg University, 3Department of Medical Oncology,NCT and Heidelberg University Hospital

Chapters

  • 00:04Title
  • 00:47Generation of Recombinant Virus Particles
  • 02:47Collection and Propagation of Virus Particles
  • 05:11Viral Titer Evaluation
  • 06:59Determining Replicative and Cytotoxic Capacities of Viral Vectors Encoding Immunomodulators
  • 08:03Assessment of Immunological Functionality of Isolated Virus-encoded Immunomodulators from Infected Cells
  • 10:00Results: Evaluation of Tumor-targeted Immunomodulation by Paramyxoviruses
  • 11:37Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a detailed workflow for the generation and ex vivo characterization of oncolytic viruses for expression of immunomodulators, using measles viruses encoding bispecific T cell engagers as an example. Application and adaptation to other vector platforms and transgenes will accelerate the development of novel immunovirotherapeutics for clinical translation.

Tags

Keywords: ParamyxovirusesTumor-targeted ImmunomodulationOncolytic VectorsReverse Genetic SystemMeasles VirusBispecific T Cell EngagerVirus PropagationSyncytia FormationVirus RescueTransfectionMV Producer CellsReporter Gene ExpressionVirus Harvest

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