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Cancer Research

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Paramyxoviruses for Tumor-targeted Immunomodulation

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Article DOI: 10.3791/58651-v 12:42 min January 7th, 2019
January 7th, 2019



This protocol describes a detailed workflow for the generation and ex vivo characterization of oncolytic viruses for expression of immunomodulators, using measles viruses encoding bispecific T cell engagers as an example. Application and adaptation to other vector platforms and transgenes will accelerate the development of novel immunovirotherapeutics for clinical translation.



Paramyxoviruses Tumor-targeted Immunomodulation Oncolytic Vectors Reverse Genetic System Immune Signatures Virus Propagation Syncytia Formation Recombinant Immunomodulatory Vectors Oncolytic Measles Vaccine Vector Bispecific T Cell Engager Transfection Measles Virus Producer Cells Recombinant DNA Antigenome Liposomal Transfection Reagent
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