Cryostat Microtomy
Cryostat Microtomy - Related Videos
06:06
A Simple Dewar/Cryostat for Thermally Equilibrating Samples at Known Temperatures for Accurate Cryogenic Luminescence Measurements
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2016
A simple liquid nitrogen Dewar/cryostat apparatus comprised of a small fused silica optical Dewar, a thermocouple, and a charge-coupled device (CCD) spectrograph are described. The experiments for which this Dewar/cryostat is designed require fast sample loading, freezing, and alignment, accurate and stable sample temperatures, and small size/portability.
07:48
Staining Protocols for Human Pancreatic Islets
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Cited by 28 •
2012
This video demonstrates procedures for characterization of human pancreatic islets using hematoxylin and eosin (H&E) and immunohistochemistry (IHC). Pancreatic sections from head, body, and tail regions are stained by both H&E and IHC to determine islet endocrine composition (insulin, glucagon, and pancreatic polypeptide), cell replication (Ki67), and inflammatory infiltrates (H&E, CD3). The uncinate region is localized using IHC for pancreatic polypeptide.
02:37
Frozen Mouse Brain Tissue Sectioning: A Procedure to Obtain Thin Frozen Tissue Sections from Frozen Murine Brain Tissue
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2023
In this video, we demonstrate the sectioning of a frozen mouse brain tumor tissue using a cryostat. The frozen brain tissue sections so obtained are stored at low temperatures until further analysis.
08:12
Thin Sectioning of Slice Preparations for Immunohistochemistry
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Cited by 7 •
2007
The present method allows reproducible cryostat sectioning of small, difficult-to-manage, tissue pieces, such as biopsies and brain slices. We utilize a simple aluminum freezing stage to facilitate handling of tissue and a standard cryostat to routinely produce 5-10 micron serial sections from 400 micron thick brain slices.
05:03
Laser Capture Microdissection to Isolate Purkinje Cells from a Human Post-Mortem Cerebellum Tissue
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2025
This video demonstrates how to isolate Purkinje cells from a human post-mortem cerebellar tissue using laser capture microdissection.
FAQs
Rapid freezing can preserve antigenicity, meaning the ability of tissue targets to retain detectable molecular features. This matters when routine processing could alter those features, because subsequent staining and microscopic analysis may no longer reflect their original distribution. In practice, researchers can use frozen sections to examine molecular markers alongside tissue structure.
The cryostat’s temperature control, specimen stabilization, and sharp blade each support section production. Temperature control keeps the frozen specimen in the cutting environment, stabilization holds it in position, and the blade permits successive sections to be collected. Together, these features provide material for staining or microscopy and help preserve interpretable tissue features.
Spatial localization shows where immune cells, pathogens, and host proteins occur within tissue rather than reporting their presence alone. Examining these distributions can connect cellular positioning with disease processes and tissue responses. This makes section-based microscopy useful for relating molecular or cellular markers to the surrounding tissue structure.
A basic workflow starts with rapidly freezing the tissue, placing it in a temperature-controlled cryostat, stabilizing the specimen, and advancing it against a sharp blade. Researchers then collect successive thin sections for staining or microscopic analysis. This sequence supports examination of both tissue structure and molecular markers while retaining features that may be affected by routine processing.
Collected sections can be subjected to staining and microscopic analysis, including immunohistochemistry. These approaches allow researchers to examine tissue structure and assess the distribution of selected molecular markers. In immunology and infection studies, the resulting images can help reveal immune cells, pathogens, or host proteins within their tissue context.
The method is especially useful when investigators need to connect tissue organization with immune or infectious processes. It supports localization of immune cells, pathogens, and host proteins, while frozen-section preparation can help preserve antigenicity. Consequently, it contributes to immunohistochemistry, diagnostic investigation, and spatial analysis of tissue responses in disease research.