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Staining Protocols for Human Pancreatic Islets
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Staining Protocols for Human Pancreatic Islets

Article DOI: 10.3791/4068
May 23rd, 2012 •
1, 1, 1, 1, 1
1Department of Pathology, Immunology, and Laboratory Medicine, University of Florida

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May 23rd, 2012

This video demonstrates procedures for characterization of human pancreatic islets using hematoxylin and eosin (H&E) and immunohistochemistry (IHC). Pancreatic sections from head, body, and tail regions are stained by both H&E and IHC to determine islet endocrine composition (insulin, glucagon, and pancreatic polypeptide), cell replication (Ki67), and inflammatory infiltrates (H&E, CD3). The uncinate region is localized using IHC for pancreatic polypeptide.

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