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DNA Transfektion av däggdjurs Skelettmuskulatur hjälp In Vivo Elektroporation
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DNA Transfektion av däggdjurs Skelettmuskulatur hjälp In Vivo Elektroporation

Article DOI: 10.3791/1520-v • 15:56 min • October 19th, 2009
October 19th, 2009 •
1, 1, 1, 1
1Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles

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Vi beskriver detaljerade förfaranden för effektiv transfektion av plasmid-DNA i fibrerna av mul muskler av levande möss med elektroporation och den efterföljande visualisering av protein uttryck med hjälp av fluorescensmikroskopi.

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Cellbiologi elektroporation skelettmuskel plasmider protein uttryck mus två-photon mikroskopi fluorescens transgena
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