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Bioengineering

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JoVE Journal Bioengineering
Roterende Cell Culture Systems for Human Cell Culture
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Roterende Cell Culture Systems for Human Cell Culture: Human trofoblasten celler som en model

Article DOI: 10.3791/3367-v • 06:54 min • January 18th, 2012
January 18th, 2012 •
*1, *1,2, 3, 1, 1
1Department of Microbiology and Immunology, Tulane University Medical School, 2Physician/Scientist Program, Tulane University Medical School, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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Traditionel, todimensionale cellekultur teknikker ofte resultere i ændrede egenskaber med hensyn til differentiering markører, cytokiner og vækstfaktorer. Tre-dimensionel cellekultur i den roterende cellekultur-system (RCCS) genopretter udtryk for mange af disse faktorer, som vist her med en extravillous trofoblasten cellelinje.

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Bioteknik Extravillous trophoblasts cytotrophoblast invasion matrix metalloproteinase 3-D cellekultur RCCS ECM microcarriers
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