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Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

Article DOI: 10.3791/4134-v • 12:49 min • June 2nd, 2012
June 2nd, 2012 •
1, 1,2, 3
1Department of Cell and Developmental Biology, Center for Stem Cell Biology, Vanderbilt University Medical Center, 2Department of Pharmacology, Center for Stem Cell Biology, Vanderbilt University Medical Center, 3Vanderbilt University Medical Center

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Isolation of embryonic neural crest from the neural tube facilitates the use of in vitro methods for studying migration, self-renewal, and multipotency of neural crest.

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Neural Crest Cells Embryonic Murine Neural Tube Isolation Culture Multipotent Progenitor Population Epithelial To Mesenchymal Transition Migration Self-renewal Tissue Types Avian Neural Tube Rat Systems Explanted NC Cells Proliferation Transplantation Cellular Properties Neural Tube Explant Assay Mammalian NC Isolation Methods Post-migratory NC Progenitors
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