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Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection
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Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection

Article DOI: 10.3791/50391-v • 10:39 min • July 5th, 2013
July 5th, 2013 •
1, 1, 1, 1, 2, 1
1Rangos Research Center, Pediatric Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Pittsburgh of UPMC, 2Department of Surgery, Tufts University Medical Center

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We describe a reproducible method of preparing mouse pancreatic acinar cells from a mouse for the purpose of examining acinar cell calcium signals and cellular injury with physiologically and pathologically relevant stimuli. A method for adenoviral infection of these cells is also provided.

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Pancreatic Acinar Cells Calcium Imaging Cell Injury Measurements Adenoviral Infection Isolation Of Acinar Cells Intracellular Calcium Signals Transfection Adenoviral Constructs Lactate Dehydrogenase Leakage Acinar Cell Physiology Acinar Cell Pathology
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