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Bestemme isbindende fly av frostvæskeproteiner ved fluorescensbasert isplanaffinitet
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Bestemme isbindende fly av frostvæskeproteiner ved fluorescensbasert isplanaffinitet

Article DOI: 10.3791/51185-v • 08:46 min • January 15th, 2014
January 15th, 2014 •
1, 2, 3, 3, 4, 1
1Department of Biomedical and Molecular Sciences, Queen's University, 2National Institute of Neurological Disorders and Stroke, Porter Neuroscience Research Center, 3Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology, 4The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition, The Hebrew University of Jerusalem

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Frostvæskeproteiner (AFPer) binder seg til bestemte isplan for å forhindre eller bremse isveksten. Fluorescensbasert isplanaffinitet (FIPA) analyse er en modifikasjon av den opprinnelige isetsingsmetoden for bestemmelse av AFP-bundet isplan. AFPer er fluorescerende merket, innlemmet i makroskopiske enkeltiskrystaller og visualisert under UV-lys.

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Kjemi Utgave 83 Materialer Biovitenskap Optikk frostvæskeproteiner Is adsorpsjon Fluorescerende merking Isgitterplan isbindende proteiner Enkel iskrystall
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