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HeLa Baseret Cell ytringsfrihed Systemer til ekspression af
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Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
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HeLa Baseret Cell ytringsfrihed Systemer til ekspression af

Article DOI: 10.3791/52772-v • 09:03 min • June 10th, 2015
June 10th, 2015 •
1, 1
1Department of Biological, Geological, and Environmental Sciences, Cleveland State University

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Ekspression af malaria proteiner i cellebaserede systemer stadig en udfordring. Vi demonstrerer to trin og et skridt IVT (in vitro translation) celle gratis ekspressionssystemer til at udtrykke malaria rekombinante rhoptry proteiner fra HeLa-celler. Vi bruger en Ni-harpiks affinitet baseret rensningssystemet for at oprense rhoptry proteiner.

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Biokemi Cell fri in vitro-transskription-translation HeLa celle frie udtryk rhoptry proteiner pattedyrscelle ytringsfrihed-system, Pro Bond affinitetsoprensning
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