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HeLa Basert Cell ytringsfrihet Systemer for Expression of
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Journal (Biology)
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
Journal (Biology)
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HeLa Basert Cell ytringsfrihet Systemer for Expression of

Article DOI: 10.3791/52772-v • 09:03 min • June 10th, 2015
June 10th, 2015 •
1, 1
1Department of Biological, Geological, and Environmental Sciences, Cleveland State University

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Uttrykk for malaria proteiner i cellebaserte systemer er fortsatt utfordrende. Vi viser to trinn og ett skritt IVT (in vitro oversettelse) celle gratis ekspresjonssystemer for å uttrykke malaria rekombinante rhoptry proteiner fra HeLa-celler. Vi bruker et Ni-resin affinitet baserte rensesystem for å rense rhoptry proteiner.

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Biokjemi Cell gratis in vitro transkripsjon-oversettelse HeLa celle ytringsfrihet rhoptry proteiner pattedyrcelle ytringsfrihet system, Pro Bond affinitetsrensing
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