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Journal / Immunology and Infection / Phänotypische Charakterisierung von Makrophagen…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Phänotypische Charakterisierung von Makrophagen aus Rattenniere mittels Durchflusszytometrie
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Phänotypische Charakterisierung von Makrophagen aus Rattenniere mittels Durchflusszytometrie

Article DOI: 10.3791/54599-v • 07:14 min • October 18th, 2016
October 18th, 2016 •
1, 1, 2, 3, 2, 2, 3, 3, 3, 1, 1
1Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz, Autonoma University, 2Department of Physiology, Faculty of Medicine, Complutense University, 3Department of Immunology, Centro Nacional de Microbiologìa, Instituto de Salud Carlos III (ISCIII)

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Diese Handschrift beschreibt ein detailliertes Protokoll für phänotypische und quantitative Analyse von Makrophagen aus Rattennieren durch Durchflusszytometrie. Die resultierenden gefärbten Zellen können auch für andere Anwendungen verwendet werden, einschließlich Zellsortierung, Genexpressionsanalyse oder funktionelle Untersuchungen, wodurch die in dem experimentellen Modell erhaltenen Informationen zu erhöhen.

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Immunologie Heft 116 Makrophagen Entzündung M1 M2 Phänotypen Polarisation Niere Ratte
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