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Journal / Immunology and Infection / Fenotypisk karakterisering av makrofager från…
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Immunology and Infection

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Fenotypisk karakterisering av makrofager från råttnjure med flödescytometri
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Fenotypisk karakterisering av makrofager från råttnjure med flödescytometri

Article DOI: 10.3791/54599-v • 07:14 min • October 18th, 2016
October 18th, 2016 •
1, 1, 2, 3, 2, 2, 3, 3, 3, 1, 1
1Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz, Autonoma University, 2Department of Physiology, Faculty of Medicine, Complutense University, 3Department of Immunology, Centro Nacional de Microbiologìa, Instituto de Salud Carlos III (ISCIII)

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Detta manuskript beskriver ett detaljerat protokoll för fenotypisk och kvantitativ analys av residenta makrofager från råttnjurar med flödescytometri. De resulterande färgade celler kan också användas för andra tillämpningar, inklusive cellsortering, genuttryck analys eller funktionella studier, vilket ökar den information som erhållits i den experimentella modellen.

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Immunologi Makrofager inflammation M1 M2 fenotyper Polarisation Kidney Råtta
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