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Genetics

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JoVE Journal Genetics
Generation von einem Gen gestört Streptococcus Mutans Belastung ohne Gene Klonen
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Generation von einem Gen gestört Streptococcus Mutans Belastung ohne Gene Klonen

Article DOI: 10.3791/56319-v • 12:06 min • October 23rd, 2017
October 23rd, 2017 •
1, 1, 1,2,3, 1
1Department of Translational Research, Tsurumi University School of Dental Medicine, 2Endowed Department of International Oral Health Science (affiliated with Department of Translational Research), Tsurumi University School of Dental Medicine, 3Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University

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Wir beschreiben eine einfache, schnelle und relativ kostengünstige Methode zur Erzeugung von gen gestört Streptococcus Mutans Stämme; Diese Technik kann für die Generierung von gen gestört Stämme verschiedener Arten angepasst werden.

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Genetik Ausgabe 128 gen Störung DNA-Konstrukt Polymerase-Kettenreaktion besser gekleideteres Design Antibiotikaresistenz-Marker-gen Streptococcus Mutans Elektroporation homologe Rekombination
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