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Journal / Immunology and Infection / Overuttryck och rening av människa
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Overuttryck och rening av människa
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Overuttryck och rening av människa

Article DOI: 10.3791/56430-v • 07:35 min • August 3rd, 2017
August 3rd, 2017 •
*1, *1, 2, 2, 1,3, 1,3, 2, 2,4, 2
1Sackler Faculty of Medicine, Tel Aviv University, 2Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 3Department of Ophthalmology, Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University, 4Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University
* These authors contributed equally

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Ett enkelt protokoll för överuttryck och rening av kodonoptimerad human cis- fenyltransferas under icke-denaturerande betingelser från Escherichia Coli , beskrivs tillsammans med en enzymatisk aktivitetsanalys. Detta protokoll kan generaliseras för produktion av andra cis- prenyltransferasproteiner i kvantitet och kvalitet lämplig för mekanistiska studier.

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Immunologi utgåva 126 kodonoptimering heterolog överuttryckning proteinrening prenyltransferas dehydrodolichyldifosfat-syntas polyprenyl
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