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Journal / Developmental Biology / Levende cel beeldvorming van chromosoom…
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Developmental Biology

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JoVE Journal Developmental Biology
Levende cel beeldvorming van chromosoom segregatie tijdens de mitose
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Levende cel beeldvorming van chromosoom segregatie tijdens de mitose

Article DOI: 10.3791/57389-v • 06:39 min • March 14th, 2018
March 14th, 2018 •
1, 2, 1
1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research, US Food and Drug Administration

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Dit protocol beschrijft een gemakkelijke en handige methode om te etiketteren en visualiseren van levende chromosomen in mitotische cellen met behulp van Histone2B-GFP BacMam 2.0 label en een draaiende schijf confocale microscopie systeem.

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Ontwikkelingsbiologie kwestie 133 chromosoom segregatie celcyclus controle spindel vergadering checkpoint levende cel imaging nocodazole muis embryonale fibroblasten eiwit fosfatase 2A Histone2B-GFP BacMam 2.0
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