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Escherichia coli-basierte zellfreie Proteinsynthese: Protokolle für eine robuste, flexible und zugängliche Plattform-Technologie

Article DOI: 10.3791/58882-v • 09:45 min • February 25th, 2019
February 25th, 2019 •
*1,2, *2,3, 4,5,6,7, 2,3, 2,3
1Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, 2Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, 3Department of Chemistry and Biochemistry, California Polytechnic State University, 4Department of Chemical and Biological Engineering, Northwestern University, 5Chemistry of Life Processes Institute, Northwestern University, 6Center for Synthetic Biology, Northwestern University, 7Interdisciplinary Biological Sciences Program, Northwestern University
* These authors contributed equally

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Dieses Protokoll beschreibt die Schritte, die Kosten und die notwendige Ausrüstung für E. Colizu generieren-basierte Zellextrakte und in-vitro- Protein Synthesereaktionen innerhalb von 4 Tagen oder weniger zu implementieren. Um die flexiblen Charakter dieser Plattform für breite Anwendungen nutzen zu können, diskutieren wir Reaktionsbedingungen, die angepasst und optimiert werden können.

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Chemie Ausgabe 144 zellfreien Protein-Synthese GFP in-vitro- Protein-Expression Hochdurchsatz-Protein Synthese TX-TL synthetische Biologie zellfreie metabolic engineering CFME
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