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Genetics

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Forbedre små RNA-SEQ: mindre bias og bedre påvisning av 2 '-O-metyl RNAs

Article DOI: 10.3791/60056-v • 08:49 min • September 16th, 2019
September 16th, 2019 •
1, 1, 1
1Institute for Integrative Biology of the Cell, UMR9198, CNRS CEA Univ Paris-Sud, Université Paris-Saclay

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Vi presenterer en detaljert liten RNA bibliotek oppreisning protokoll med mindre bias enn standard metoder og en økt følsomhet for 2 '-O-methyl RNAs. Denne protokollen kan følges ved hjelp av hjemmelagde reagenser for å spare kostnader eller bruke kits for enkelhets skyld.

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Genetikk liten RNA liten RNA-SEQ bias bibliotek forberedelser neste generasjons sekvensering NGS 2 '-O-metyl (2 '-OMe) RNA anlegg microRNA Plant miRNA
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