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Murine Precision-Cut Leverskiver som en Ex Vivo-modell av leverbiologi
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Murine Precision-Cut Leverskiver som en Ex Vivo-modell av leverbiologi

Article DOI: 10.3791/60992-v • 12:36 min • March 14th, 2020
March 14th, 2020 •
*1, *1, 2,3, 1,4,5, 6, 7, 8,9, 2,10, 1,4
1Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, 2School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, 3School of Veterinary and Life Sciences, Murdoch University, 4School of Medicine, The University of Queensland, 5Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, 6School of Medicine, Griffith University, 7School of Biological Sciences, Queen's University Belfast, 8Department of Gastroenterology & Hepatology, Fiona Stanley and Fremantle Hospital Group, 9School of Medical and Health Sciences, Edith Cowan University, 10Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences, University of Western Australia
* These authors contributed equally

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Denne protokollen gir en enkel og pålitelig metode for produksjon av levedyktige presisjonskuttede leverskiver fra mus. Ex vivo vevsprøvene kan opprettholdes under laboratorievevskulturforhold i flere dager, noe som gir en fleksibel modell for å undersøke leverens patolog.

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Medisin Utgave 157 lever skiver vibratome ex vivo hepatocytter vev kultur stellate celler
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