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Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin

Article DOI: 10.3791/63449-v • 08:07 min • March 24th, 2023
March 24th, 2023 •
1, 1, 2, 3,4, 3
1Centro de Investigaciones en Óptica A.C. (CIO), 2Division of Sciences and Engineering, Department of Chemical, Electronic, and Biomedical Engineering, Universidad de Guanajuato, 3Institute of Physiology, Benemérita Universidad Autónoma de Puebla, 4Dirección General de Desarrollo Internacional, Benemérita Universidad Autónoma de Puebla

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In this article, we present a protocol to detect microtubule-loaded oligodendrocytes in a model of tubulinopathy through a simple, innovative second harmonic generation microscopy approach.

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Label-Free Non-Linear Optics Second-Harmonic Generation Imaging Tubulin-dependent Defects Central Myelin Diagnostic Imaging Tubulin Beta-4A Taiep Animal Model Tubulinopathies Pulse Laser Tissular Microtubules Optical Detection Path
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