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Journal / Genetics / 使用慢病毒介导的CRISPR /…
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Genetics

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JoVE Journal Genetics
使用慢病毒介导的CRISPR / Cas9基因编辑开发敲除肌肉细胞系
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使用慢病毒介导的CRISPR / Cas9基因编辑开发敲除肌肉细胞系

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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该协议描述了如何使用CRISPR / Cas9生成敲除肌母细胞，从引导RNA的设计到细胞克隆和敲除克隆的表征。

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遗传学，第184期，
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