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Journal / Immunology and Infection / Analys av T-cellreceptorinducerad kalciuminflöde…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Analys av T-cellreceptorinducerad kalciuminflöde i primära murina T-celler med fullspektrumflödescytometri
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Analys av T-cellreceptorinducerad kalciuminflöde i primära murina T-celler med fullspektrumflödescytometri

Article DOI: 10.3791/64526-v • 10:01 min • December 16th, 2022
December 16th, 2022 •
1, 1, 1
1Department of Immunology and Microbiology, University of Colorado-Anschutz School of Medicine

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Kalciuminflöde, ett mått på T-cellsignalering, är ett effektivt sätt att analysera svar på T-cellreceptorstimulering. Detta protokoll för multiplexering av Indo-1 med paneler av antikroppar riktade mot cellytemolekyler utnyttjar de mycket flexibla funktionerna hos fullspektrumflödescytometri.

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Immunologi och infektion utgåva 190
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