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Immunology and Infection
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Propagation des virus et quantification colorimétrique cellulaire

Article DOI: 10.3791/64578-v • 07:26 min • April 7th, 2023
April 7th, 2023 •
1, 1, 2, 1, 1
1Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE), Universiti Malaya, 2Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya

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Le présent protocole décrit la propagation du virus Zika (ZIKV) dans les cellules rénales de singes verts d’Afrique et la quantification du virus Zika à l’aide de méthodes d’immunodétection colorimétrique cellulaires dans des formats à 24 puits et 96 puits (haut débit).

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Immunologie et infection Numéro 194 Virus Zika (ZIKV) Flavivirus Anomalies cérébrales congénitales Syndrome de Guillain-Barrã© Virologie Test de formation de foyer (FFA) Antigène viral Technique d’immunomarquage de la peroxydase Format 24 puits Format 96 puits Optimisation de la taille des foyers Cellules véro Incubation Processus de coloration Fixation cellulaire Perméabilisation Blocage Liaison aux anticorps Substrat de peroxydase
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