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Agrobacterium tumefaciens-gemedieerde genetische transformatie van smalbladige weegbree
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Agrobacterium tumefaciens-gemedieerde genetische transformatie van smalbladige weegbree

Article DOI: 10.3791/64777-v • 07:50 min • March 17th, 2023
March 17th, 2023 •
1, 1, 1, 2, 1, 3, 4, 1
1Department of Agronomy, Center for Plant Biology, Purdue University, 2Department of Biology, Purdue University, 3Department of Horticulture, Faculty of Agricultural Sciences, University of the Punjab, 4Department of Botany and Plant Pathology, Center for Plant Biology, Purdue University

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Vanwege de veelzijdige toepassing als modelsoort in verschillende vakgebieden, is er behoefte aan een toolkit voor genetische transformatie in smalbladige weegbree (Plantago lanceolata). Hier wordt met behulp van Agrobacterium tumefaciens-gemedieerde transformatie een protocol gepresenteerd dat resulteert in stabiele transgene lijnen met een transformatie-efficiëntie van 20%.

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