Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Ashley H. Ramsawhook; Lara C. Lewis; Maria Eleftheriou; Abdulkadir Abakir; Paulina Durczak; Robert Markus; Seema Rajani; Nicholas R.F. Hannan; Beth Coyle; Alexey Ruzov

doi:10.3791/56318

ジャーナル

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遺伝学

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DNA 変更染色: 共焦点画像の数値解析

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

JoVE Journal
遺伝学

This content is Free Access.

JoVE Journal 遺伝学

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

DNA 変更染色: 共焦点画像の数値解析

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9,588 Views

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09:42 min

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September 07, 2017

DOI:

10.3791/56318-v

DOI:

10.3791/56318-v

•

09:42 min

September 07, 2017

•

3 Views

Ashley H. Ramsawhook*¹, Lara C. Lewis*¹, Maria Eleftheriou¹, Abdulkadir Abakir¹, Paulina Durczak¹, Robert Markus², Seema Rajani², Nicholas R.F. Hannan¹, Beth Coyle³, Alexey Ruzov¹

¹Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences,University of Nottingham, ²School of Life Sciences Imaging (SLIM), School of Life Sciences,University of Nottingham, ³Children’s Brain Tumour Research Centre, School of Medicine, QMC,University of Nottingham

筆記録

概要

新たに発見された 5-メチルシトシン (oxi mCs), 5-ヒドロキシメチルシトシン (5hmC), 5-formylcytosine (5 fC) の酸化フォームと 5-carboxylcytosine (5caC) は、独自の機能的役割の異なる DNA 変更を表す場合があります。ここの可視化から oxi mCs の空間分布、信号強度プロファイルとの共存の半定量的なワークフローを説明します。