Name: 薬用植物におけるトロパンアルカロイドの定量のための効率的かつ迅速なHPLC-QQQ-MS法 (動画) | JoVE Journal
Uploaded: 2026-06-12T13:00:00.000+00:00
Duration: 9 min 10 s
Description: 学ぶHPLC-QQQ-MS法によるAnisodus tanguticusにおけるトロパンアルカロイドの迅速かつ正確な定量法。従来のHPLCの感度限界を克服し、ヒオスシアミン、アニソダミン、スコポラミンの高精度定量が可能です。

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</ol>

```html
<table><tbody><tr><td>Agilent MassHunter software</td><td>Agilent Technologies</td><td></td><td></td></tr><tr><td>Agilent ZORBAX SB-C18 column</td><td>Agilent Technologies</td><td>880975-902</td><td>C18 reversed-phase column, 4.6 &times; 250 mm, 5 &mu;m</td></tr><tr><td>アニソダミン ヒドロブロミド</td><td>成都グリップバイオテクノロジー株式会社</td><td>101-31-5</td><td>HPLC>98%</td></tr><tr><td>酢酸</td><td>シグマ・アルドリッチ</td><td>5.33002</td><td></td></tr><tr><td>凍結乾燥機</td><td>EYELA、東京、日本</td><td>FDU-2110</td><td></td></tr><tr><td>高速万能グラインダー</td><td>太石機器有限公司、中国天津市</td><td>6010210100</td><td>FW-100、24,000 rpm、100 g容量、80メッシュ</td></tr><tr><td>ヒオシアミン</td><td>成都グリップバイオテクノロジー株式会社</td><td>55449-49-5</td><td>HPLC>98%</td></tr><tr><td>メタノール</td><td>Thermo Fisher Scientific</td><td>022909.K2</td><td></td></tr><tr><td>マイクロポラス膜0.22&mu;m</td><td>ミリポア</td><td>SLGV033RB</td><td></td></tr><tr><td>Milli-Q Direct 8水システム</td><td>ミリポアシグマ、バーリントン、MA、米国</td><td>ZRQSVP0JP</td><td></td></tr><tr><td>スコポラミン ヒドロブロミド</td><td>成都グリップバイオテクノロジー株式会社</td><td>114-49-8</td><td>HPLC>98%</td></tr></tbody></table>
```

薬用植物におけるトロパンアルカロイドの定量のための効率的かつ迅速なhplc-qqq-ms法

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants

HPLC-QQQ-MS クロマトグラフィー分離およびTAの同定 標準混合物とA.tanguticus根抽出物の分析から得られる典型的なMRMクロマトグラムは図3および図4に示されています。最適化されたHPLC条件下では、ヒヨスチアミン、アニソダミン、スコポラミンの3つの標的トロパンアルカロイドすべてが20分以内に成功して分離され、開発された方法の高い効率が実証されました。スコポラミンとアニソダミン間の分解能(Rs)は8.67、アニソダミンとヒヨスチアミン間の分解能は11.58でした。すべての解像度値は基準分離基準の1.5を大幅に上回り、クロマトグラフィー干渉がないことを示し、正確な定量解析に適していることを裏付けました。すべての化合物は鋭く対称的なピーク形状を示し、マトリックス干渉を最小限に抑えつつ優れたクロマトグラフィー性能を示しました。各TAの同定は、(1)本物の標準と一致する保持時間(±±...

薬用植物におけるトロパンアルカロイドの定量のための効率的かつ迅速なHPLC-QQQ-MS法

Anisodus tanguticus is an important Tibetan medicinal plant. The tropane alkaloids (TAs), such as hyoscyamine, anisodamine, and scopolamine, exhibit ...

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インビボヌクレオシド/ヌクレオチドの植物定量の正確かつ再現可能な方法をここに記載する。この方法では、HPLC-MS/MS...

HPLC-MS/MSによるヌクレオシド/ヌクレオチド含有量測定のための植物サンプル調製

本プロトコルは、茶園のPA産生雑草からの茶サンプル中のピロリジジンアルカロイド(PA)の汚染について説明しています。...

茶サンプル中のピロリジジンアルカロイド汚染の発生源と経路

ここでは、動物由来の医薬品中のトリメチルアミン(TMA)の測定に適したヘッドスペースガスクロマトグラフィー-タンデム四重極質量分析(HS-GC-MS/MS)法について説明します。プロトコルには、サンプルの前処理、ヘッドスペース処理、分析条件、方法論的検証、および動物由来医薬品中のTMAの測定が含まれます。...

ヘッドスペースガスクロマトグラフィー-タンデム四重極質量分析法による動物由来医薬品におけるトリメチルアミン検出の改良技術

トロパンアルカロイドの迅速な質量分析(MS)/質量分析(MS)ベースのアノテーションと分類の方法を提示し、トロパン含有サンプルの予備的な再現性の除去と単離のための新規アルカロイドの発見の両方に役立ちます。...

天然物研究における液体クロマトグラフィータンデム質量分析の応用:ケーススタディとしてのトロパンアルカロイド

このプロトコルは、最近発表された欧州委員会規則(EU)2023/915に準拠して、食品中の麦角アルカロイドの存在を決定するための検証済みの液体クロマトグラフィー-イオン移動度-高分解能質量分析法を提供します。...

液体クロマトグラフィートラップイオン移動度分析飛行時間型質量分析による食品マトリックス中の制御された麦角アルカロイドの検出

このプロトコルは、試薬の精製、反応条件、バイオコンジュゲートの精製およびバイオコンジュゲートの特徴付けを含む、マレイミドにタンパク質を含有するシステインのバイオコンジュゲーションのために必要な重要な手順を詳しく説明します。...

タンパク質のバイオコンジュゲートの合成<em&gt;経由で</em&gt;システイン - マレイミドケミストリー

私たちは、5-ノルボルネン-2-カルボン酸と5-ビニル-2-ノルボルネンの触媒挿入重合により、非常に高いガラス転移温度を持つ機能性ポリマーを形成することを説明しています。...

機能性ノルボルネンの触媒挿入重合のためのシンプルで効率的なプロトコル

125 I標識アジドの合成と銅のないクリック反応を用いて、dibenzocyclooctyne（DBCO） -基コンジュゲート、13-nmサイズの金ナノ粒子の放射性標識のための詳細な手順が記載されています。...

使用した金ナノ粒子の効率的な放射性標識のために最適化されたプロトコル<sup&gt; 125</sup&gt; I標識アジド補綴グループ

学ぶHPLC-QQQ-MS法によるAnisodus tanguticusにおけるトロパンアルカロイドの迅速かつ正確な定量法。従来のHPLCの感度限界を克服し、ヒオスシアミン、アニソダミン、スコポラミンの高精度定量が可能です。

薬用植物におけるトロパンアルカロイドの定量のための効率的かつ迅速なHPLC-QQQ-MS法

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