遺伝学、第124号、CRISPR / Cas9、相同性駆動修復、非相同末端結合、ドットブロット、クローン選択、スクリーニング Protocols and Video Articles

Research

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CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Authors

Author

Kyle E. Simpson¹, Shoaib Faizi¹, Ravichandra Venkateshappa¹, Mandy Yip¹, Raj Johal¹, Damon Poburko¹, Yen May Cheng¹, Diana Hunter¹, Eric Lin¹, Glen F. Tibbits¹, Thomas W. Claydon¹

Institution

¹Department of Biomedical Physiology and Kinesiology, Simon Fraser University

Journal (Biology)

| Methods Collections

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Authors

Author

Daniel E. Bauer*^1,2,3, Matthew C. Canver*¹, Stuart H. Orkin^1,2,3,4

Institution

¹Harvard Medical School, ²Division of Hematology/Oncology, Boston Children's Hospital, ³Department of Pediatric Oncology, Dana-Farber Cancer Institute, ⁴Howard Hughes Medical Institute

Journal (Biology)

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Authors

Author

Linyuan Ma*¹, Lydia Jang*¹, Jian Chen², Jun Song¹, Dongshan Yang¹, Jifeng Zhang¹, Y. Eugene Chen¹, Jie Xu¹

Institution

¹Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, ²Celetrix LLC

Journal (Genetics)

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Authors

Author

Andi M. Wilson^1,2, Brenda D. Wingfield^1,2

Institution

¹Department of Biochemistry, Genetics & Microbiology, University of Pretoria, ²Forestry & Agricultural Biotechnology Institute (FABI), University of Pretoria

Journal (Genetics)

Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Authors

Author

Na Tang^1,2, Yaoyao Zhang¹, Miriam Pedrera¹, Pengxiang Chang¹, Susan Baigent¹, Katy Moffat¹, Zhiqiang Shen², Venugopal Nair¹, Yongxiu Yao¹

Institution

¹The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, ²Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

Journal (Immunology and Infection)

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Education

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CRISPR

JoVE Core (Core: 생물학)

CRISPR and crRNAs

JoVE Core (Core: 분자생물학)

CRISPR

JoVE Core (Core: 분자생물학)

Uniform Distribution

JoVE Core (Core: 통계학)

Genome Editing

Science Education (Advanced Biology)

Chromatin Immunoprecipitation- ChIP

JoVE Core (Core: 분자생물학)

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