In Vivo Development
In Vivo Development - Related Videos
08:39
Ex vivo Culturing of Whole, Developing Drosophila Brains
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Cited by 16 •
2012
This article describes a method by which one can mimic in vivo development of the Drosophila mushroom body in an ex vivo culture system.
05:53
In vivo Electroporation of Developing Mouse Retina
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Cited by 33 •
2011
A method for the incorporation of plasmid DNA into murine retinal cells for the purpose of performing either gain- or loss of function studies in vivo is presented. This method capitalizes on the transient increase in permeability of cell plasma membranes induced by the application of an external electrical field.
08:33
An Ex vivo Culture System to Study Thyroid Development
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Cited by 14 •
2014
This protocol describes dissection of mouse embryonic thyroid anlagen and the culture of explants on semiporous filters or on microscopy plastic slides. This system is ideal to study morphogenetic or differentiation events occurring during thyroid development of wild type or knockout embryos, and is amenable to gain- and loss-of-function experiments.
11:51
Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
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Cited by 9 •
2009
Mesoscopic fluorescence tomography operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The technique is based on multi-projection illumination and a photon transport description. We demonstrate in-vivo whole-body 3D visualization of the morphogenesis of GFP-expressing wing imaginal discs in Drosophila melanogaster.
22:02
Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
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Cited by 23 •
2012
The mouse inner ear is a placode-derived sensory organ whose developmental program is elaborated during gestation. We define an in utero gene transfer technique consisting of three steps: mouse ventral laparotomy, transuterine microinjection, and in vivo electroporation. We use digital video microscopy to demonstrate the critical experimental embryological techniques.
FAQs
In vivo development is shaped by native physiological signals and interactions present in the intact nervous system. These cues influence how neural stem and progenitor cells proliferate, migrate, and differentiate rather than acting in isolation. Their combined effects help determine where cells settle, what identities they acquire, and how developing neural tissue becomes organized.
Gene expression, cell-cell signaling, and the tissue environment provide distinct but connected layers of regulation. Gene expression influences cellular programs, while neighboring cells and surrounding tissue contribute signals that guide developmental behavior. Examining these factors together is important because neural maturation reflects coordinated interactions rather than a single controlling variable.
Progression from axon guidance to synapse formation links cellular development with circuit construction. Axon guidance helps developing neurons establish appropriate connections, while synapse formation creates points of communication within those circuits. Studying both stages in vivo helps investigators relate local cellular events to neural circuit assembly and, ultimately, to brain structure and function.
An in vivo development study can organize its analysis around proliferation, migration, differentiation, axon guidance, and synapse formation. Researchers can interpret these outcomes alongside gene expression, cell-cell signaling, and tissue-level context. This framework connects observed cellular changes to broader questions about how neural circuits assemble within the intact nervous system.
This approach is especially relevant when a question depends on native interactions, such as how neural cells respond to surrounding tissue or how circuits form. It supports investigation of neurodevelopmental disorders, injury and repair, and therapies under biologically realistic conditions. These applications benefit from preserving the physiological context that shapes neural development.
They can evaluate therapies in biologically realistic settings while relating treatment-associated changes to neural tissue, circuit assembly, or repair. The surrounding physiological context helps connect observed effects with processes such as cell maturation and neural organization. This makes the approach useful for determining whether an intervention engages relevant developmental or repair mechanisms in the nervous system.