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Pulse-chase analyse van N-gebonden suikerketens van glycoproteïnen in zoogdiercellen

Article DOI: 10.3791/1899-v • 10:18 min • April 27th, 2010
April 27th, 2010 •
1, 1, 1, 1, 1
1Department of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University

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We beschrijven een methode voor de analyse van de verandering van N-gekoppelde glycanen door het vroege leven van glycoproteïnen na hun biosynthese in zoogdiercellen. Dit wordt bereikt door pulse-chase analyse van metabolisch gelabeld glycanen, enzymatische vrijlating uit glycoproteïnen en onderzoek door HPLC.

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Cellular Biology N-gekoppelde oligosaccharide mannose-etikettering endoplasmatisch reticulum verbonden degradatie calnexin glycosylering mannosidase
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