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Expressie analyse van zoogdieren Linker-histon Subtypes

Article DOI: 10.3791/3577-v • 14:40 min • March 19th, 2012
March 19th, 2012 •
1, 1, 1, 1
1School of Biology and the Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology

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We beschrijven een reeks testen om de expressie niveaus van H1 linker histonen te analyseren. mRNA van de individuele H1 genen worden kwantitatief gemeten door een willekeurige primer op basis reverse transcriptie gevolgd door real-time PCR, terwijl eiwit kwantificering van H1 histonen wordt bereikt met behulp van HPLC-analyse.

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Genetica H1 linker histonen histon H1 subtypes chromatine RT-PCR HPLC genexpressie
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