Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Evaluating Quantitative Tumor Cell Killing by… / Preparation of Plate for Fluorescent Imaging and…
JoVE Journal
Cancer Research

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.

JoVE Journal Cancer Research
Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells (Video) | JoVE
Cytokinesis-Block Micronucleus Assay on Irradiated Blood Cell Culture Prepared from Cryopreserved Whole Blood Samples (Video) | JoVE
Journal (Cancer Research)
Cytokinesis-Block Micronucleus Assay on Irradiated Blood Cell Culture Prepared from Cryopreserved Whole Blood Samples (Video) | JoVE
Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
Journal (Cancer Research)
Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
 

Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells (Video) | JoVE

Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

Article DOI: 10.3791/200793-v • 04:00 min • October 27th, 2023
October 27th, 2023 •
*1,2,3, *1, 4, 2, 1, 4,5,6, 1,2,3
1Department of Radiation Oncology, University of Kansas Cancer Center, 2Department of Cancer Biology, University of Kansas Cancer Center, 3BioEngineering Program, University of Kansas, 4Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, 5University of Kansas Cancer Center, 6Kansas Institute for Precision Medicine, University of Kansas Medical Center
* These authors contributed equally

Tags

Rapid In Vitro Cytotoxicity Evaluation Jurkat Chimeric Antigen Receptor Fluorescent Imaging CAR T Cells Targeting Domain Single Chain Variable Fragment Intra-chain Linker Hinge Domain Transmembrane Domain Costimulatory Domain CAR Construct CAR-mediated Killing CAR-T Cells Time-consuming Process Expensive Process Testing Constructs Time And Material Investment Platform Hinge-optimized CAR Constructs Jurkat Cells Lentivirus Uptake CAR Transduction Fluorescent Imager Cytolysis PBMC-derived T Cells
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter