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iClip - Transkriptom-weiten Mapping von Protein-RNA Wechselwirkungen mit Individual Nucleotide Resolution

Article DOI: 10.3791/2638-v • 10:45 min • April 30th, 2011
April 30th, 2011 •
1, 2, 3, 3, 1, 3, 4, 2, 1
1Laboratory of Molecular Biology, Medical Research Council - MRC, 2European Bioinformatics Institute, EMBL Heidelberg, 3Computer and Information Science, University of Ljubljana, 4Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute

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Die räumliche Anordnung der RNA-bindenden Proteinen auf eine Abschrift ist eine wichtige Determinante der post-transkriptionellen Regulation. Deshalb entwickelten wir individuelle-Nukleotid-Auflösung UV-Vernetzung und Immunpräzipitation (iClip), die eine präzise genomweite Kartierung der Bindungsstellen eines RNA-bindenden Proteins erlaubt.

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Cellular Bioloy RNA-Biochemie Transkriptom Systembiologie RNA-bindende Protein
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