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Morpholinos in vivo Elektroporasyon Yetişkin Zebra balığı Retina

Article DOI: 10.3791/3603-v • 06:55 min • December 27th, 2011
December 27th, 2011 •
1, 2,3, 2,3
1Departments of Anatomy and Cell Biology and Ophthalmology, Wayne State University School of Medicine, 2Department of Biological Sciences, University of Notre Dame, 3Center for Zebrafish Research, University of Notre Dame

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Intravitreal antisens morpholinos enjekte ve retina içine electroporating koşullu yetişkin zebrafish retinanın bir hedef protein ifade demonte bir yöntem açıklanmıştır. Ortaya çıkan protein yenileyici veya sağlam retina proteinin rolünü test edilmesine imkan sağlayan birkaç gün, nakavt edilir.

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Gelişimsel Biyoloji Sayı 58 Elektroporasyon morfolino Zebra balığı retina rejenerasyon
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