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JoVE Journal
Biology

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使用具体远藤和exoglycosidases糖基化蛋白质的鉴定和表征
 
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使用具体远藤和exoglycosidases糖基化蛋白质的鉴定和表征

Article DOI: 10.3791/3749-v 09:54 min December 26th, 2011
December 26th, 2011

챕터

요약

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使用特定的糖苷酶,以消除通过SDS - PAGE的糖蛋白的糖是一种有价值的的方法来检测蛋白质样品聚糖修改初始糖生物学研究的是一个不错的选择。以下deglycosylation变化可以检测凝胶的流动性的变化或与多糖敏感的试剂染色。

Tags

分子生物学,58期,糖蛋白,N -糖链,O型多糖,PNGase F,O -糖苷酶,deglycosylation,糖苷酶
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