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In vitro analys av PDZ-beroende Signaling CFTR makromolekylära komplex
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In vitro analys av PDZ-beroende Signaling CFTR makromolekylära komplex

Article DOI: 10.3791/4091-v • 10:05 min • August 13th, 2012
August 13th, 2012 •
1, 1, 1,2,3
1Department of Biochemistry & Molecular Biology, Wayne State University School of Medicine, 2Cardiovascular Research Institute, Wayne State University School of Medicine, 3Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine

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Cystisk fibros transmembran konduktans-regulator (CFTR), en epitelial kloridkanal, har rapporterats interagera med olika proteiner och reglera viktiga cellulära processer, däribland de CFTR PDZ-motivet-medierade interaktioner har dokumenterats väl. Detta protokoll beskriver metoder vi utvecklat för att montera en PDZ-beroende CFTR makromolekylära signalering komplex

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Biokemi molekylärbiologi kemi CFTR makromolekylära komplex protein interaktion PDZ schavotten protein epitelceller cystisk fibros
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