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Journal / Immunology and Infection / Überwachen von Änderungen in Membran-Polarity,…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Überwachen von Änderungen in Membran-Polarity, Membrane Integrity und intrazelluläre Ionenkonzentrationen in
A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice
Journal (Immunology and Infection)
A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice
Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses
Journal (Immunology and Infection)
Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses
 
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Überwachen von Änderungen in Membran-Polarity, Membrane Integrity und intrazelluläre Ionenkonzentrationen in

Article DOI: 10.3791/51008-v • 11:17 min • February 17th, 2014
February 17th, 2014 •
*1, *1, 1, 1,2,3
1Department of Microbiology and Immunology, University at Buffalo, State University of New York, 2Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, State University of New York, 3New York State Center of Excellence in Bioinformatics and Life Sciences, University at Buffalo, State University of New York
* These authors contributed equally

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Anders als bei Eukaryonten zu sehen, gibt es nur wenig Untersuchungen, die detailliert Membrandepolarisation und Ionenkonzentrationsänderungen in Bakterien, in erster Linie ihrer geringen Größe macht herkömmliche Messverfahren schwierig. Hier haben wir ausführlich Protokolle zur Überwachung solcher Veranstaltungen in der deutlichen Gram-positive Erreger Streptococcus pneumoniae Verwendung Fluoreszenztechniken.

Tags

Immunologie , Pneumokokken Potential-sensitiven Farbstoffen DiBAC Propidiumiodid acetoxymethyl (AM) Ester- Blasensprung Ionentransport bakterielle Ionenkonzentrationen ionensensitiven Fluoreszenz
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