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Quantitative Proteomics Mit reduktive Dimethylierung Stabile Isotope Labeling

Article DOI: 10.3791/51416-v • 11:53 min • July 1st, 2014
July 1st, 2014 •
1,2,3, 4
1CEA, DSV, IG, Genoscope, 2CNRS-UMR8030, Évry, France, 3Université d'Évry Val d'Essonne, 4Massachusetts General Hospital Cancer Center

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Stabile Isotopenmarkierung von Peptiden durch reduktive Dimethylierung (Redi-Markierung) ist eine schnelle, kostengünstige Strategie für genaue Massenspektrometrie-basierte quantitative Proteomik. Hier zeigen wir ein robustes Verfahren für die Aufbereitung und Analyse von Proteingemischen mit Hilfe der redi Ansatz, der auf fast jedem Probentyp angewandt werden kann.

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Chemie Heft 89 quantitative Proteomik Massenspektrometrie stabiles Isotop reduktive Dimethylierung Peptid-Kennzeichnung LC-MS/MS
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