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Kwantitatieve Proteomics behulp Reductieve dimethylering voor stabiele isotopen Labeling
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Kwantitatieve Proteomics behulp Reductieve dimethylering voor stabiele isotopen Labeling

Article DOI: 10.3791/51416-v • 11:53 min • July 1st, 2014
July 1st, 2014 •
1,2,3, 4
1CEA, DSV, IG, Genoscope, 2CNRS-UMR8030, Évry, France, 3Université d'Évry Val d'Essonne, 4Massachusetts General Hospital Cancer Center

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Stabiele isotoop labeling van peptiden door reductieve dimethylering (Redi etikettering) is een snelle, goedkope strategie voor nauwkeurige massa-spectrometrie gebaseerde kwantitatieve proteomics. Hier laten we een robuuste werkwijze voor de bereiding en analyse van eiwitmengsels met de Redi aanpak die kan worden toegepast op vrijwel elk type monster.

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Chemie kwantitatieve proteomics massaspectrometrie stabiele isotopen reductieve dimethylering peptide etikettering LC-MS/MS
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