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JoVE Journal
Immunology and Infection

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人类单核双梯度离心法和其分化为巨噬细胞的特富龙涂层的细胞培养袋隔离
 
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人类单核双梯度离心法和其分化为巨噬细胞的特富龙涂层的细胞培养袋隔离

Article DOI: 10.3791/51554-v 09:32 min September 9th, 2014
September 9th, 2014

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我们提出了一个简单而有效的协议对人类巨噬细胞的产生。血沉棕黄层用双密度梯度离心法处理,并分离单核细胞,然后分化为巨噬细胞中的特氟龙涂层的细胞培养袋。这最大限度地提高巨噬细胞的产量,并促进细胞收集在后续实验。

Tags

免疫学,第91,巨噬细胞,单核细胞,分离,PBMC中,密度梯度,分化,特氟龙涂层的细胞培养袋
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