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In-vitro-Pankreas Organogenese von Dispersed embryonalen Maus-Vorläufer

Article DOI: 10.3791/51725-v • 08:35 min • July 19th, 2014
July 19th, 2014 •
*1, *1, *2, 1,2
1Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences, Swiss Institute for Experimental Cancer Research, 2DanStem, University of Copenhagen
* These authors contributed equally

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Die in diesem Protokoll beschriebenen dreidimensionalen Kulturverfahren rekapituliert Bauchspeicheldrüse Entwicklung von verteilten embryonalen Maus-Pankreasvorläuferzellen, einschließlich ihrer erheblichen Ausweitung, Differenzierung und Morphogenese in einem verzweigten Organ. Dieses Verfahren ist zugänglich Bildgebung, funktionelle Störungen und Manipulationen der Nische.

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Entwicklungsbiologie Bauchspeicheldrüse Vorläufer Branching Epithel Entwicklung Orgel Kultur 3D-Kultur Diabetes Differenzierung Morphogenese Zellorganisation Beta Cell.
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