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Immunology and Infection

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Biolistic Transformasjon av en Fluorescent Tagged Gene inn i Opportunistic Fungal Patogen

Article DOI: 10.3791/52666-v • 07:32 min • March 19th, 2015
March 19th, 2015 •
1, 2, 1, 1
1Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center (EPIC), Clemson University, 2Department of Biology, Western Carolina University

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Biolistic transformasjon er en metode som brukes til å generere stabil integrering av DNA inn i genomet til de opportunistiske patogener Cryptococcus neoformans gjennom homolog rekombinasjon. Vi vil demonstrere biolistic transformasjon av en konstruksjon, som har genet som koder acetat kinase kondensert til den fluorescerende tag mCherry inn C. neoformans.

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Molecular Biology genet avbrudd, Biolistic levering acetat kinase overlapping PCR fluorescens
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