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Isolamento e Análise de Citometria de Fluxo infiltrantes Glioma Células Mononucleares do Sangue Periférico

Article DOI: 10.3791/53676-v • 12:52 min • November 28th, 2015
November 28th, 2015 •
1,2, 1,2, 1,2
1Department of Neurosurgery, University of Michigan, 2Department of Cell and Developmental Biology, University of Michigan

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Apresentado aqui é um método simples para o isolamento e análise de citometria de fluxo de células mononucleares de sangue periférico que se infiltram no glioma que produz dados quantitativos tempo-dependente do número e estado de activação das células do sistema imunológico que entram no microambiente do tumor cerebral precoce.

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Imunologia Edição 105 glioma as células mononucleares do sangue periférico (PBMC) a galectina-1 (Gal-1) GL26-Cit-gal1i GL26-Cit-NT GR-1 natural killer (NK)
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