A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication

Jason D. Domogauer; Sonia M. de Toledo; Edouard I. Azzam

doi:10.3791/54429

Journal

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암 연구학

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종양 미세 환경의 모방 : 세포 간 통신을 농축 세포 인구를 생성 및 조사를위한 간단한 방법

A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication

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JoVE 신문 암 연구학

A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication

종양 미세 환경의 모방 : 세포 간 통신을 농축 세포 인구를 생성 및 조사를위한 간단한 방법

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10,258 Views

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09:52 min

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September 20, 2016

DOI:

10.3791/54429-v

DOI:

10.3791/54429-v

•

09:52 min

September 20, 2016

•

10223 Views

Jason D. Domogauer¹, Sonia M. de Toledo¹, Edouard I. Azzam¹

¹Department of Radiology, New Jersey Medical School,Rutgers University

Summary

We adapted a permeable microporous membrane insert to mimic the tumor microenvironment (TME). The model consists of a mixed cell culture, allows simplified generation of highly enriched individual cell populations without using fluorescent tagging or cell sorting, and permits studying intercellular communication within the TME under normal or stress conditions.