Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Charlotte S.L. Bailey; Robert A. Bone; Philip J. Murray; J. Kim Dale

doi:10.3791/55127

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자세한 공간 식지도에서 동적 식 데이터의 시간적 순서

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

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Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

자세한 공간 식지도에서 동적 식 데이터의 시간적 순서

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5,872 Views

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11:52 min

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February 09, 2017

DOI:

10.3791/55127-v

DOI:

10.3791/55127-v

•

11:52 min

February 09, 2017

•

3 Views

Charlotte S.L. Bailey*¹, Robert A. Bone*¹, Philip J. Murray², J. Kim Dale³

¹The Danish Stem Cell Center (DanStem),University of Copenhagen, ²Division of Mathematics,University of Dundee, ³Division of Cell and Developmental Biology, College of Life Sciences,University of Dundee

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Summary

The segmentation clock drives oscillatory gene expression across the pre-somitic mesoderm (PSM). Dynamic Notch activity is key to this process. We use imaging and computational analyses to extract temporal dynamics from spatial expression data to demonstrate that Delta ligand and Notch receptor expression oscillate in the vertebrate PSM.