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Journal / Developmental Biology / Kultur von Adult transgener Zebrafische Retinal…
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Developmental Biology

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Kultur von Adult transgener Zebrafische Retinal Explantate für das Live-Cell-Imaging von Multiphotonen-Mikroskopie
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Kultur von Adult transgener Zebrafische Retinal Explantate für das Live-Cell-Imaging von Multiphotonen-Mikroskopie

Article DOI: 10.3791/55335-v • 11:55 min • February 24th, 2017
February 24th, 2017 •
1, 1, 1,2, 1
1Department of Biological Sciences, University of Notre Dame, 2Department of Neurosurgery, Mayo Clinic

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Zebrabärbling retinale Regeneration hat meist unter Verwendung von Festnetzhäuten untersucht. Allerdings dynamische Prozesse wie interkinetic Kern Migration treten während der regenerativen Reaktion und erfordern Live-Cell-Imaging die zugrunde liegenden Mechanismen zu untersuchen. Hier beschreiben wir Kultur und Abbildungsbedingungen Interkinetic Kern Migration (INM) in Echtzeit unter Verwendung von Multiphotonen-Mikroskopie zu überwachen.

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Entwicklungsbiologie Heft 120 Retinal Explantation Multiphotonen-Mikroskopie Live-Cell-Imaging Zebrabärbling interkinetic Kern Migration Kultur Regeneration Müller Glia Mitose Geschwindigkeit
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