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Journal / Biology / In situ Immunfluoreszenz Färbung der Autophagie…
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In situ Immunfluoreszenz Färbung der Autophagie in Muskelstammzellen

Article DOI: 10.3791/55908-v • 08:35 min • June 12th, 2017
June 12th, 2017 •
1,2, 3, 4, 1,2
1Department of Medicine, Institute of Translational Pharmacology, Italian National Research Council, 2Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences, University of Modena and Reggio Emilia

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Aktive Autophagie ist mit produktiven Muskelregeneration verbunden, die für die Muskelstammzell-Aktivierung (MuSC) essentiell ist. Hier stellen wir ein Protokoll für die in situ- Detektion von LC3, einen Autophagie-Marker in MyoD-positiven MuSCs von Muskelgewebeabschnitten von Kontroll- und verletzten Mäusen zur Verfügung.

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Zelluläre Biologie Ausgabe 124 Autophagie Muskelstammzellen (Satelliten) Muskelregeneration LC3 MYOD Muskelgewebeabschnitte
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