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Journal / Developmental Biology / De aorta Ring mede cultuur Assay: Een handig…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
De aorta Ring mede cultuur Assay
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De aorta Ring mede cultuur Assay: Een handig hulpmiddel om te beoordelen van het potentieel van de Angiogenic van mesenchymale stromale cellen In Vitro

Article DOI: 10.3791/56083-v • 10:04 min • September 18th, 2017
September 18th, 2017 •
1,2, 1, 1,2, 1,2,3,4,5
1Create Fertility Centre, 2Department of Physiology, University of Toronto, 3Department of Obstetrics and Gynecology, University of Toronto, 4Department of Medical Sciences, University of Toronto, 5Department of Obstetrics and Gynecology, Women's College Hospital

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Hier presenteren we een nieuwe toepassing van de bepaling van de aorta ring waar prelabelled mesenchymale cellen zijn mede gekweekte met rat aorta afkomstige endothelial netwerken. Deze nieuwe methode kunt visualisatie van mesenchymale stromale cellen (MSCs) homing en integratie met endothelial netwerken, kwantificering van netwerkeigenschappen en evaluatie van MSC immunophenotypes en gen-expressie.

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Ontwikkelingsbiologie kwestie 127 angiogenese aorta ring assay cel migratie endotheel netwerken mesenchymale stromale cellen fysieke interacties paracrine factoren gerelateerde cellen navelstreng
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