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Neuroscience

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Imagerie de l’activité neuronale dans le Cortex somatosensoriel primaire utilisant Thy1-GCaMP6s des souris transgéniques

Article DOI: 10.3791/56297-v • 07:04 min • January 7th, 2019
January 7th, 2019 •
1,2, 3, 1, 4, 1, 1
1Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Department of Anatomy and Cell Biology, Indiana University School of Medicine, 2Department of Spinal Cord Injury and Repair, Trauma and Orthopedics Institute of Chinese PLA, General Hospital of Jinan Military Region, 3Department of Orthopedics, Shandong Cancer Hospital, Shandong University, 4Department of Neurobiology, Institute of Basic Medical Sciences, Academy Military Medical Sciences

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Nous décrivons une procédure expérimentale pour mesurer l’activité neuronale à travers les fenêtres optiques doubles au-dessus corticies somatosensoriel primaire bilatérale (S1) en Thy1-GCaMP6s des souris transgéniques à l’aide de 2 photons (2p) microscopie in vivo.

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Neurosciences numéro 143 GCaMP6s des souris transgéniques l’imagerie In vivo fenêtre crânienne cortex somatosensoriel 2-photon imaging dynamique du Calcium
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