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Ein Graben/Tunneling Test zur Erkennung von Hypoxie in Drosophila Melanogaster Larven

Article DOI: 10.3791/57131-v • 09:04 min • March 27th, 2018
March 27th, 2018 •
1,2, 1, 1
1Department of Biosciences, Rice University, 2Yale Medical School

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Das Protokoll beschreibt einen einfache Assay Drosophila Melanogaster Larven zu identifizieren, die Hypoxie unter normalen atmosphärischen Sauerstoffniveaus durchleben. Dieses Protokoll ermöglicht hypoxische Larven zu unterscheiden von anderen Mutanten, die überlappende Phänotypen wie Trägheit oder langsames Wachstum zeigen.

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Verhalten Ausgabe 133 D. Melanogaster Larve Hypoxie Nahrung zu wühlen Substrat tunneling Larven Wachstum Luftröhre larval Fortbewegung atemlos uninflatable
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